proteing plus Search Results


klebsiella pneumoniae  (Vakzine Projekt Management GmbH)
90
Vakzine Projekt Management GmbH klebsiella pneumoniae
Klebsiella Pneumoniae, supplied by Vakzine Projekt Management GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/proteing+plus/klebsiella+pneumoniae/pm24932553-143-2-1
Average 90 stars, based on 1 article reviews
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protev plus  (Promega)
90
Promega protev plus
Protev Plus, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/proteing+plus/protev+plus/us10774364-1570-38-40
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protev plus enzyme  (Promega)
90
Promega protev plus enzyme
Protev Plus Enzyme, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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protev plus protease  (Promega)
90
Promega protev plus protease
Protev Plus Protease, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vwr extra pure  (Chem Impex International)
95
Chem Impex International vwr extra pure
Vwr Extra Pure, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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proteing plus agarose beads  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology proteing plus agarose beads
Proteing Plus Agarose Beads, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/proteing+plus/Protein+G+PLUS-Agarose/pmc02727665-145-2-5
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protev plus protease v6101  (Promega)
90
Promega protev plus protease v6101
Protev Plus Protease V6101, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/proteing+plus/tev+protease/pmc09672857-169-18-21
Average 90 stars, based on 1 article reviews
protev plus protease v6101 - by Bioz Stars, 2026-08
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proteing plus/proteina-agarose beads  (PROTEINA Co Ltd)
90
PROTEINA Co Ltd proteing plus/proteina-agarose beads
Proteing Plus/Proteina Agarose Beads, supplied by PROTEINA Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tobacco etch virus (tev) protease  (Promega)
90
Promega tobacco etch virus (tev) protease
( a ) Schematic of sample preparation. Dicer was constructed with Flag, <t>TEV</t> and AP tags, which were used for immunoprecipitation, elution and biotinylation, respectively. <t>Dicer</t> <t>proteins</t> were biotinylated in HEK 293 cells. The proteins were immunoprecipitated using Flag-antibody beads. The proteins were eluted out of the beads via TEV cleavage. ( b ) Western blotting of Dicer IPs. Dicer proteins were expressed without and with Myc-TRBP and were pulled down using Flag beads (left). In vitro cleavage of pre-let-7a-1 3′2nt (right). Mock is a negative control with Flag-mCherry IPs. ( c ) Schematic of single-molecule immobilization. Dicer IPs were conjugated to a polymer-coated surface via NeutrAvidin–biotin interaction. Contaminant proteins were washed away before 200 pM Cy5-labelled pre-let-7a-1 3′2nt was introduced. Interactions between the surface-immobilized Dicer complexes with Cy5-labelled pre-miRNA were visualized through total internal reflection fluorescence (TIRF) microscopy. Dots in the CCD (charge-coupled device) image reflect docking of pre-let-7a-1 3′2nt to individual Dicer complexes. The CCD image illustrated the binding events over 25 × 25 μm 2 field of view. Scale bar, 5 μm. ( d ) The CCD images in the left illustrate the stable docking of Cy-5-labelled pre-let-7a-1 3′2nt to surface-immobilized Dicer complexes in absence (upper panels) and presence of 1 μM competitor tRNA (bottom panels). The histogram in the right quantifies the inhibition of the pre-miRNA-binding activity due to the presence of 1 μM competitor tRNA. Error is the s.d. obtained from 10 different fields of view in three independent experiments. Scale bar, 5 μm.
Tobacco Etch Virus (Tev) Protease, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/proteing+plus/tobacco+etch+virus+protease/pmc05155159-156-15-27
Average 90 stars, based on 1 article reviews
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normalmouse igg plus proteing agarose  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology normalmouse igg plus proteing agarose
( a ) Schematic of sample preparation. Dicer was constructed with Flag, <t>TEV</t> and AP tags, which were used for immunoprecipitation, elution and biotinylation, respectively. <t>Dicer</t> <t>proteins</t> were biotinylated in HEK 293 cells. The proteins were immunoprecipitated using Flag-antibody beads. The proteins were eluted out of the beads via TEV cleavage. ( b ) Western blotting of Dicer IPs. Dicer proteins were expressed without and with Myc-TRBP and were pulled down using Flag beads (left). In vitro cleavage of pre-let-7a-1 3′2nt (right). Mock is a negative control with Flag-mCherry IPs. ( c ) Schematic of single-molecule immobilization. Dicer IPs were conjugated to a polymer-coated surface via NeutrAvidin–biotin interaction. Contaminant proteins were washed away before 200 pM Cy5-labelled pre-let-7a-1 3′2nt was introduced. Interactions between the surface-immobilized Dicer complexes with Cy5-labelled pre-miRNA were visualized through total internal reflection fluorescence (TIRF) microscopy. Dots in the CCD (charge-coupled device) image reflect docking of pre-let-7a-1 3′2nt to individual Dicer complexes. The CCD image illustrated the binding events over 25 × 25 μm 2 field of view. Scale bar, 5 μm. ( d ) The CCD images in the left illustrate the stable docking of Cy-5-labelled pre-let-7a-1 3′2nt to surface-immobilized Dicer complexes in absence (upper panels) and presence of 1 μM competitor tRNA (bottom panels). The histogram in the right quantifies the inhibition of the pre-miRNA-binding activity due to the presence of 1 μM competitor tRNA. Error is the s.d. obtained from 10 different fields of view in three independent experiments. Scale bar, 5 μm.
Normalmouse Igg Plus Proteing Agarose, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/proteing+plus/normal+mouse+IgG/10__1074_slash_jbc__m701719200-124-25-29
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normalmouse igg plus proteing agarose - by Bioz Stars, 2026-08
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protev plus 20200703  (Promega)
90
Promega protev plus 20200703
( a ) Schematic of sample preparation. Dicer was constructed with Flag, <t>TEV</t> and AP tags, which were used for immunoprecipitation, elution and biotinylation, respectively. <t>Dicer</t> <t>proteins</t> were biotinylated in HEK 293 cells. The proteins were immunoprecipitated using Flag-antibody beads. The proteins were eluted out of the beads via TEV cleavage. ( b ) Western blotting of Dicer IPs. Dicer proteins were expressed without and with Myc-TRBP and were pulled down using Flag beads (left). In vitro cleavage of pre-let-7a-1 3′2nt (right). Mock is a negative control with Flag-mCherry IPs. ( c ) Schematic of single-molecule immobilization. Dicer IPs were conjugated to a polymer-coated surface via NeutrAvidin–biotin interaction. Contaminant proteins were washed away before 200 pM Cy5-labelled pre-let-7a-1 3′2nt was introduced. Interactions between the surface-immobilized Dicer complexes with Cy5-labelled pre-miRNA were visualized through total internal reflection fluorescence (TIRF) microscopy. Dots in the CCD (charge-coupled device) image reflect docking of pre-let-7a-1 3′2nt to individual Dicer complexes. The CCD image illustrated the binding events over 25 × 25 μm 2 field of view. Scale bar, 5 μm. ( d ) The CCD images in the left illustrate the stable docking of Cy-5-labelled pre-let-7a-1 3′2nt to surface-immobilized Dicer complexes in absence (upper panels) and presence of 1 μM competitor tRNA (bottom panels). The histogram in the right quantifies the inhibition of the pre-miRNA-binding activity due to the presence of 1 μM competitor tRNA. Error is the s.d. obtained from 10 different fields of view in three independent experiments. Scale bar, 5 μm.
Protev Plus 20200703, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/proteing+plus/protev+plus+20200703/10__1091_slash_mbc__e20___05___0290-263-24-26
Average 90 stars, based on 1 article reviews
protev plus 20200703 - by Bioz Stars, 2026-08
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two arm randomized design comparing dbt plus dm  (Impeto Medical Inc)
86
Impeto Medical Inc two arm randomized design comparing dbt plus dm
( a ) Schematic of sample preparation. Dicer was constructed with Flag, <t>TEV</t> and AP tags, which were used for immunoprecipitation, elution and biotinylation, respectively. <t>Dicer</t> <t>proteins</t> were biotinylated in HEK 293 cells. The proteins were immunoprecipitated using Flag-antibody beads. The proteins were eluted out of the beads via TEV cleavage. ( b ) Western blotting of Dicer IPs. Dicer proteins were expressed without and with Myc-TRBP and were pulled down using Flag beads (left). In vitro cleavage of pre-let-7a-1 3′2nt (right). Mock is a negative control with Flag-mCherry IPs. ( c ) Schematic of single-molecule immobilization. Dicer IPs were conjugated to a polymer-coated surface via NeutrAvidin–biotin interaction. Contaminant proteins were washed away before 200 pM Cy5-labelled pre-let-7a-1 3′2nt was introduced. Interactions between the surface-immobilized Dicer complexes with Cy5-labelled pre-miRNA were visualized through total internal reflection fluorescence (TIRF) microscopy. Dots in the CCD (charge-coupled device) image reflect docking of pre-let-7a-1 3′2nt to individual Dicer complexes. The CCD image illustrated the binding events over 25 × 25 μm 2 field of view. Scale bar, 5 μm. ( d ) The CCD images in the left illustrate the stable docking of Cy-5-labelled pre-let-7a-1 3′2nt to surface-immobilized Dicer complexes in absence (upper panels) and presence of 1 μM competitor tRNA (bottom panels). The histogram in the right quantifies the inhibition of the pre-miRNA-binding activity due to the presence of 1 μM competitor tRNA. Error is the s.d. obtained from 10 different fields of view in three independent experiments. Scale bar, 5 μm.
Two Arm Randomized Design Comparing Dbt Plus Dm, supplied by Impeto Medical Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/proteing+plus/arm+comparing+dbt+design+dm+plus+randomized+two/pm39162938-39-6-18
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two arm randomized design comparing dbt plus dm - by Bioz Stars, 2026-08
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Image Search Results


( a ) Schematic of sample preparation. Dicer was constructed with Flag, TEV and AP tags, which were used for immunoprecipitation, elution and biotinylation, respectively. Dicer proteins were biotinylated in HEK 293 cells. The proteins were immunoprecipitated using Flag-antibody beads. The proteins were eluted out of the beads via TEV cleavage. ( b ) Western blotting of Dicer IPs. Dicer proteins were expressed without and with Myc-TRBP and were pulled down using Flag beads (left). In vitro cleavage of pre-let-7a-1 3′2nt (right). Mock is a negative control with Flag-mCherry IPs. ( c ) Schematic of single-molecule immobilization. Dicer IPs were conjugated to a polymer-coated surface via NeutrAvidin–biotin interaction. Contaminant proteins were washed away before 200 pM Cy5-labelled pre-let-7a-1 3′2nt was introduced. Interactions between the surface-immobilized Dicer complexes with Cy5-labelled pre-miRNA were visualized through total internal reflection fluorescence (TIRF) microscopy. Dots in the CCD (charge-coupled device) image reflect docking of pre-let-7a-1 3′2nt to individual Dicer complexes. The CCD image illustrated the binding events over 25 × 25 μm 2 field of view. Scale bar, 5 μm. ( d ) The CCD images in the left illustrate the stable docking of Cy-5-labelled pre-let-7a-1 3′2nt to surface-immobilized Dicer complexes in absence (upper panels) and presence of 1 μM competitor tRNA (bottom panels). The histogram in the right quantifies the inhibition of the pre-miRNA-binding activity due to the presence of 1 μM competitor tRNA. Error is the s.d. obtained from 10 different fields of view in three independent experiments. Scale bar, 5 μm.

Journal: Nature Communications

Article Title: TRBP ensures efficient Dicer processing of precursor microRNA in RNA-crowded environments

doi: 10.1038/ncomms13694

Figure Lengend Snippet: ( a ) Schematic of sample preparation. Dicer was constructed with Flag, TEV and AP tags, which were used for immunoprecipitation, elution and biotinylation, respectively. Dicer proteins were biotinylated in HEK 293 cells. The proteins were immunoprecipitated using Flag-antibody beads. The proteins were eluted out of the beads via TEV cleavage. ( b ) Western blotting of Dicer IPs. Dicer proteins were expressed without and with Myc-TRBP and were pulled down using Flag beads (left). In vitro cleavage of pre-let-7a-1 3′2nt (right). Mock is a negative control with Flag-mCherry IPs. ( c ) Schematic of single-molecule immobilization. Dicer IPs were conjugated to a polymer-coated surface via NeutrAvidin–biotin interaction. Contaminant proteins were washed away before 200 pM Cy5-labelled pre-let-7a-1 3′2nt was introduced. Interactions between the surface-immobilized Dicer complexes with Cy5-labelled pre-miRNA were visualized through total internal reflection fluorescence (TIRF) microscopy. Dots in the CCD (charge-coupled device) image reflect docking of pre-let-7a-1 3′2nt to individual Dicer complexes. The CCD image illustrated the binding events over 25 × 25 μm 2 field of view. Scale bar, 5 μm. ( d ) The CCD images in the left illustrate the stable docking of Cy-5-labelled pre-let-7a-1 3′2nt to surface-immobilized Dicer complexes in absence (upper panels) and presence of 1 μM competitor tRNA (bottom panels). The histogram in the right quantifies the inhibition of the pre-miRNA-binding activity due to the presence of 1 μM competitor tRNA. Error is the s.d. obtained from 10 different fields of view in three independent experiments. Scale bar, 5 μm.

Article Snippet: For the tandem purification, proteins were eluted out of the beads by site-specific cleavage with tobacco etch virus (TEV) protease (0.05 U μl −1 ; ProTEV Plus, Promega) at 30 °C for 90 min.

Techniques: Sample Prep, Construct, Immunoprecipitation, Western Blot, In Vitro, Negative Control, Polymer, Fluorescence, Microscopy, Binding Assay, Inhibition, Activity Assay